Coding

Part:BBa_K4879002

Designed by: Rohaneshwar Manikandan   Group: iGEM23_IISER-Pune-India   (2023-10-08)


CvFAP

The fatty acid photodecarboxylase from Chlorella variabilis. [1]

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Usage and Biology

The fatty acid photodecarboxylase, found mainly in the chloroplasts of microalga Chlorella variabilis, catalyses the decarboxylation of free fatty acids into n-alkanes or n-alkenes in the presence of blue light. The reaction is initiated by electron abstraction from the fatty acid to a photo-excited cofactor flavin adenine dinucleotide (FAD).

The direct conversion of free fatty acids (FFAs) into n-alkanes and n-alkenes is an essential feature of our project as it bypasses a series of more expensive, intensive and CO2 emitting chemical hydroprocessing steps in fuel (hydrocarbon) production.


The protein structure of CvFAP, as predicted by AlphaFold


Design

The transcriptional unit for CvFAP (BBa_K4879008) is designed with the coding sequence flanked by the TEF1 promoter (BBa_K3629001) upstream and the XPR2 terminator (BBa_K3629004) downstream.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 259
    Illegal PstI site found at 691
    Illegal PstI site found at 958
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 259
    Illegal PstI site found at 691
    Illegal PstI site found at 958
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1424
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 259
    Illegal PstI site found at 691
    Illegal PstI site found at 958
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 259
    Illegal PstI site found at 691
    Illegal PstI site found at 958
    Illegal NgoMIV site found at 1579
    Illegal AgeI site found at 1555
  • 1000
    COMPATIBLE WITH RFC[1000]


References

1. Bruder, S., Moldenhauer, E.J., Lemke, R.D. et al. Drop-in biofuel production using fatty acid photodecarboxylase from Chlorella variabilis in the oleaginous yeast Yarrowia lipolytica. Biotechnol Biofuels 12, 202 (2019). https://doi.org/10.1186/s13068-019-1542-4

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